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A previously developed polyclonal antiserum to double-stranded RNA was used to demonstrate the presence of polyanionic sites (D+, Cl-) on the membrane of the intra-luminal vesicles and the plasma membrane of early spermatids in mice. These sites probably contain the ultrastructural analogue of the unique RNA present in spermatocytes and spermatids and localized in the cytoplasm and nucleus of spermatocytes. These findings suggest that protein synthetic systems are initiated in spermatid cells during spermatogenesis in mice and that RNA may be present in the cytoplasm and nucleus of spermatocytes.Pulmonary fibrosis is a fatal lung disease characterized by excess fibroblastic cell (myofibroblast) and collagen deposition in the interstitium of the lung parenchyma. There are three main categories of lung fibrosis; pulmonary alveolar proteinosis (PAP), pulmonary fibrosis and pulmonary inflammatory disease. Pulmonary alveolar proteinosis is characterized by accumulation of lipoproteinaceous material in the alveoli. Pulmonary fibrosis and pulmonary inflammatory disease are both characterized by altered lung architecture, such as accumulation of fibroblast derived fibrils, marked pulmonary inflammation and progressive lung scarring. The molecular mechanisms involved in the fibroblast to myofibroblast transition in pulmonary fibrosis are unknown. This is an important issue for the development of new therapies for the treatment of pulmonary fibrosis. A better understanding of how fibroblasts go from quiescent normal cells to myofibroblasts with increased contractile activity and extracellular matrix production in the fibrotic interstitium is needed for the development of new treatments for the treatment of pulmonary fibrosis. Our hypothesis is that activated macrophages play a key role in the fibroblast to myofibroblast transition in
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